Purification of Total RNA from Mammalian Cells and Tissues

A rapid protocol for purification of total RNA for tissues collected from pigs at a slaughterhouse.

Since RNA extraction is a crucial step in many molecular techniques, the protocols for sample collection and RNA purification need to be adapted to optimize their performance when samples are collected from animals at commercial facilities. Here we provide an RNA purification protocol for animal tissues collected from slaughterhouses. This protocol, modified from other techniques, uses TRI...

Protein purification from whole tissues . Fatemeh Mehrpooyan*

Cytoplasmic RNA extraction from fresh and frozen mammalian tissues.

The quality of collections of expressed sequence tags andfull-length cDNAs is adversely affected by the presence of "junk" clones derivedfrom unspliced or partially spliced RNAs present in conventional total RNA preparations. One can overcome this problem by using intact cytoplasmic RNA to create cDNA libraries, but the methods in the literature that describe the preparation of RNA only work we...

Identification and Purification of two Mammalian Nourotoxins from Iranian Scorpion (Buthotus schach) Venom

  Scorpion venoms contain of variety of peptides toxic to mammals ، insects and crustaceans. Toxic peptides are the main factors in scorpion venom causing toxicity, (their amount being 1-3%of total venom.). Most of the scorpion toxins have been isolated from the venoms of scorpions in the family Buthidae. The scorpion Buthotus Schach belonging to the Buthidae family is widely found in the weste...

Purification and properties of dihydrofolate reductase from cultured mammalian cells.

Dihydrofolate reductase was purified quickly and simply from small quantities of cultured mammalian cells by affinity chromatography. On gel electrophoresis of the purified enzyme, multiple bands of activity resulted from enzyme-buffer interaction at low but not high buffer concentration. A Ferguson plot (Ferguson, 1964) showed that this heterogeneity was due to a charge difference with no alte...